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. 2009 Nov;41(11):2295–2301. doi: 10.1016/j.biocel.2009.05.012

Fig. 1.

Fig. 1

Infection of H9C2 cells with gly193M-LDH/ΔM-LDH has a dominant-negative effect on M-LDH physically associated with KATP channel subunits. (A) Original Western blot of anti-Kir6.2 precipitate of H9C2 control cells and cells infected with gly193M-LDH/ΔM-LDH (the size of M-LDH is 36 kDa; similar results were obtained in three blots). (B) LDH assay with anti-Kir6.2 immunoprecipitate of membrane fraction of control cells (control) and cells infected with gly193M-LDH/ΔM-LDH (similar results were obtained in three experiments). (C) Current–voltage relationships in control cells and cells infected with gly193M-LDH/ΔM-LDH filled with pipette solution containing ATP (3 mM) plus pyruvate (20 mM) plus NADH (20 mM). Each point represent mean (n = 5–6).