Fig. 5.

Effect of nicotine on IκB-α degeradation. Mesencephalic cells were treated with or without nicotine (1 μM) for different time intervals as described in materials and methods. Cells were washed with PBS and cell extracts were made in PBS by homogenization. Equal quantity of protein (75 μg) was separated on sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to nylon membranes and probed with IκB-α antibodies washed thrice with PBS and incubated with second antibody horse radish peroxidase and bands were analyzed by chemiluminiscence kit.