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. Author manuscript; available in PMC: 2009 Oct 6.
Published in final edited form as: Mol Cell Biochem. 2006 Oct 5;297(1-2):93–99. doi: 10.1007/s11010-006-9333-1

Fig. 6.

Fig. 6

Effect of nicotine on JNK activation. Cell extracts from control and nicotine treated mesencephalic cells were prepared as described in materials and methods and 300 μg of total protein extract were immuno-precipitated with anti-JNK (0.3 μg/sample) antibody, and the kinase reaction was performed using 32P-labeled ATP and GST-Jun as substrate protein. The radiolabeled GST-Jun was detected in 9% SDS-PAGE. The level of JNK was detected using 50 μg of same extract proteins and analyzed in 10% SDS-PAGE by Western blot.