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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Mol Microbiol. 2009 Jun 23;73(2):226–239. doi: 10.1111/j.1365-2958.2009.06765.x

Table 1.

Suppression of constitutive SOS expression by recA4162 and recA4164 in cis and in trans with recA730 and recA4142

Strains Chr.recAo Chr.recA Plasmid recA RFI ratioa % of cells expressing SOSC ≥ 6-fold
SS996 + + 1.0 ± 0.0 0.2 ± 0.1
SS4629 + 730 40.4 ± 7.1 99.6 ± 0.2
SS6013 + 4142 3.6 ± 0.7 7.6 ± 2.8
SS6052 + 4162 1.8 ± 0.1 3.6 ± 2.9
SS6027 + 4164 2.0 ± 0.1 2.0 ± 1.3
SS4630 + 730,4162 b 1.0 ± 0.6 0.3 ± 0.2
SS5292 + 730,4164 0.8 ± 0.2 1.0 ± 0.1
SS4976 o1403 4142 34.5 ± 1.5 100 ± 0.0
SS6089 o1403 4142,4162 1.0 ± 0.2 0.5 ± 0.4
SS6062 o1403 4142,4164 2.2 ± 0.2 1.9 ± 1.0
SS5376 + 730 pDPT429 56.1 ± 4.3 98.9 ± 1.7
SS6074 + 730 recA 48.0 ± 8.3 99.8 ± 0.2
SS6077 + 730 recA4162 7.8 ± 1.9 13.3 ± 1.5
SS6079 + 730 recA4164 7.1 ± 2.0 18.9 ± 7.3
SS5377 o1403 4142 pDPT429 34.0 ± 8.9 89.3 ± 18.5
SS6075 o1403 4142 recA 14.9 ± 2.8 81.8 ± 13.1
SS6076 o1403 4142 recA4162 7.0 ± 2.6 15.5 ± 6.7
SS6078 o1403 4142 recA4164 6.6 ± 1.3 10.1 ± 2.0
SS6118 + del 730 33.6 ± 2.7 99.2 ± 1.0
SS6086 + recA4162 730 1.6 ± 0.5 1.64 ± 1.4
SS6085 + recA4164 730 1.0 ± 0.6 1.38 ±0.5
SS6100 + del 4142 28.2 ± 1.9 81.5 ± 7.0
SS6081 + recA4162 4142 1.7 ± 0.4 3.95 ± 2.0
SS6082 + recA4164 4142 2.3 ± 0.7 2.66 ± 2.9
a

See Materials and Methods for specific protocols. All Strains were grown in minimal medium at 37°C in log phase before sampling. All numbers are the average of three independent measurements. For each strain approximately 1000–2000 cells were counted. All strains having recA4142 on the chromosome also have recAo1403. Plasmid versions of recA4142 are recAo+. RFI stand for Relative Fluorescence Intensity and is a measure of the SOSC expression.

b

recA730,4162 (recA441) are sampled at 30°C.