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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Mol Microbiol. 2009 Jun 23;73(2):226–239. doi: 10.1111/j.1365-2958.2009.06765.x

Table 5.

Strains used in this work

Strain ygaD recAo recA RecX uvrD attλ Other relevant genotype Origin of reference
CAG18491 + + + + + + metE3079::Tn10 E.coli Stock Center
DM1187 + + 730,4162 (recA441) + + + D. Mount
GY8382 + + 938::cat + + + B. Michel
JC13509a + + + + + + Lab Stock
JJC7643 + + + + 307 + yigE::cat B. Michel
K253 + + 730,4164 (recA718) + + + srlC300::Tn10 M. Volkert
KL880 + + + + 252 + B. Low
N14–4 + + + + 3 + B. Low
N3055 + + + + + + uvrA277::Tn10 R.G. Lloyd
SS391 + + 938::cat + + + GY8382→JC13509c
SS996 + + + + + Ωgfp (McCool et al., 2004)
SS1054 + + + + + + metE3079::Tn10 (Centore et al., 2009)
SS1436 + + 938::cat + + Ωgfp (McCool et al., 2004)
SS4070 + + + + + + del(mutL)100::kan (Baba et al., 2006)
SS4073 + + + + + + del(mutH)100::kan (Baba et al., 2006)
SS4539 + + + + 252 + KL880→SS1054g
SS4540 + + + + 3 + N14–4→SS1054g
SS4558 + + + + 252 Ωgfp gal-76::Tn10 SS1465→SS4539c
SS4559 + + + + 3 Ωgfp gal-76::Tn10 SS1465→SS4540c
SS4570 + + + + kan + (Baba et al., 2006)
SS4626 + + + + + Ωgfp zfj-3131::Tn10 alaS5 (Long et al., 2008)
SS4629 + + 730 + + Ωgfp (Long et al., 2008)
SS4630 + + 730,4162 + + Ωgfp DM1187→SS4626e
SS4976 kan o1403 4142 + + Ωgfp (Long et al., 2008)
SS5130 + + + + + + del(uvrB)100::kan (Baba et al., 2006)
SS5802 + + + + + Ωgfp metE3079::Tn10 CAG18491→SS996c
SS5292 + + 730,4164 + + Ωgfp srlC300::Tn10 K253→SS996c
SS5296 kan + 803,4162 + + Ωgfp Lab Stock
SS5376 + + 730 + + Ωgfp pDPT429→SS4629d
SS5377 kan o1403 4142 + + Ωgfp pDPT429→SS4976d
SS5737 + + + + 701 Ωgfp gal-76::Tn10 (Centore et al., 2009)
SS5752 + + + + + Ωgfp uvrA277::Tn10 N3055→SS996c
SS5753 + + + + 307 Ωgfp yigE::cat JJC2643→SS5802g
SS5767 + + + + + Ωgfp del(uvrB)100::kan SS5130→SS996b
SS5949 + + + + + Ωgfp del(mutL)100::kan SS4070→SS996b
SS5958 + + del(recA)100::kan + + Ωgfp SS4952→SS996b
SS6013 kan + 4142 + + Ωgfp SS6009→SS996h
SS6089 kan o1403 4142,4162 + + Ωgfp SS5323→SS996b, h
SS6027 kan + 4164 + + Ωgfp SS6026→SS996b, h
SS6052 kan + 4162 + + Ωgfp SS6430→SS4630b, h
SS6062 kan o1403 4142,4164 + + Ωgfp SS6444→SS996b, h
SS6074 + + 730 + + Ωgfp pNR122→SS4629d
SS6075 kan o1403 4142,4164 + + Ωgfp pNR122→SS4976d
SS6076 kan o1403 4142,4164 + + Ωgfp pNR123→SS4976d
SS6077 + + 730 + + Ωgfp pNR123→SS4629d
SS6078 kan o1403 4142,4164 + + Ωgfp pNR124→SS4976d
SS6079 + + 730 + + Ωgfp pNR124→SS4629d
SS6080 + + + cat + Ωgfp (Long et al., 2008)
SS6081 kan + 4162 + + Ωgfp pEL16→SS6052d
SS6082 kan + 4164 + + Ωgfp pEL16→SS6027d
SS6085 kan + 4164 + + Ωgfp pNR127→SS6027d
SS6086 kan + 4162 + + Ωgfp pNR127→SS6052d
SS6118 + + del + + Ωgfp pNR127→SS5958d
SS6100 + + del + + Ωgfp pEL16→SS5958d
SS7125 + + + + kan Ωgfp SS4570→SS996b
SS7139 + + + + del Ωgfp SS7134i
SS7434 kan 1403 4142,4162 + del Ωgfp SS5323→SS7139b
SS7449 kan 1403 4142,4162 cat + Ωgfp SS7444→SS996f
SS7450 kan 1403 4142,4162 cat del Ωgfp SS7444→SS7139f
SS7451 kan 1403 4142,4162 cat 701 Ωgfp gal-76::Tn10 SS7444→SS5737f
SS7457 kan 1403 4142,4162 cat 307 Ωgfp yigE::cat SS7444→SS5753f
SS7459 kan 1403 4142,4162 cat 252 Ωgfp SS7444→SS4558f
SS7463 + + 730,4164 + del Ωgfp srlC300::Tn10 K253→SS7139c
SS7464 + + 730,4162 + kan Ωgfp SS4570→SS4630b
SS7465 kan 1403 4142,4164 + del Ωgfp SS6444→SS7139b
SS7467 + + + cat del Ωgfp SS6080→SS7139f
SS7468 + + + cat 252 Ωgfp SS6080→SS4558f
SS7470 + + + cat 701 Ωgfp SS6080→SS7139f
SS7474 + + 730,4162 cat + Ωgfp SS7471→SS996j, f
SS7475 + + 730,4164 cat + Ωgfp srlC300::Tn10 SS7472→SS996j, f
SS7476 kan 1403 4142,4164 cat + Ωgfp SS7473→SS996j, f
SS7477 + + 730,4162 cat del Ωgfp SS7471→SS7139f
SS7478 + + 730,4164 cat del Ωgfp srlC300::Tn10 SS7472→SS7139f
SS7479 kan 1403 4142,4164 cat del Ωgfp SS7473→SS7139f
SS7480 + + + cat + Ωgfp metE3079::Tn10 CAG18491→SS6080c
SS7481 + + + cat 307 Ωgfp yigE::cat JJC7643→SS7480g
SS7485 + + 730,4164 cat 3 Ωgfp SS7472→SS4559f
SS7486 + + 730,4164 cat 701 Ωgfp SS7472→SS5737f
SS7487 + + 730,4164 cat 252 Ωgfp SS7472→SS4558f
SS7488 + + 730,4164 cat 307 Ωgfp yigE::cat srlC300::Tn10 SS7472→SS5753c
SS7489 kan 1403 4142,4162 cat 3 Ωgfp SS7444→SS4559f
SS7604 + + + + + Ωgfp del(mutH)100::kan SS4073→SS996b
SS7605 + + + + + Ωgfp del(mutS)100::kan SS4882→SS996b
SS7611 kan o1403 4142,4162 cat + Ωgfp del(mutL)100::kan SS7444→SS5949j, f
SS7612 kan o1403 4142,4162 cat + Ωgfp del(uvrB)100::kan SS7444→SS5767f
SS7613 + + 730,4164 cat + Ωgfp del(mutL)100::kan srlC300::Tn10 SS7472→SS5949j, f
SS7614 + + 730,4164 cat + Ωgfp del(uvrB)100::kan srlC300::Tn10 SS7472→SS5767f
SS7615 kan 1403 4142,4162 cat + Ωgfp del(uvrA)100::kan SS7444→SS5752f
SS7616 + + 730,4164 cat + Ωgfp del(uvrA)100::kan srlC300::Tn10 SS7472→SS5752f
SS7617 kan 1403 4142,4162 cat + Ωgfp del(mutS)100::kan SS7444→SS7605f
SS7618 + + 730,4164 cat + Ωgfp del(mutS)100::kan srlC300::Tn10 SS7472→SS7605f
SS7619 kan 1403 4142,4162 cat + Ωgfp del(mutH)100::kan SS7444→SS7604f
SS7620 + + 730,4164 cat + Ωgfp del(mutH)100::kan srlC300::Tn10 SS7472→SS7604f
a

JC13509 has the following genotype: sulB103 lacMS286 φ 80dIIlacBK1 argE3 hi-4 thi-1 xyl-5 mtl-1 rpsL31 tsx. The lacMS286φ80dIIlacBK1 code for two partial non-overlapping deletions of the lac operon (Konrad, 1977; Zieg and Kushner, 1977). Full notation for ygaD mutation is ygaD1::kan. Full notation for Ωgfp mutation is Δattλ::sulApΩgfp-mut2.

b

Select for KanR and then screen for other marker phenotypically or by PCR.

c

Select for TetR and then screen for other marker phenotypically or by PCR.

d

Select for AmpR

e

Select for AlaS+

f

Select for CatR

g

Select for Met+

h

These recAo or recA mutations were first constructed on a plasmid as described in the Materials and Methods. They were then transferred to the chromosome using the method of Datsenko and Wanner (Datsenko and Wanner, 2000) using a strain that was lexA3 malE::Tn10 in a JC13509 background with pKD46 encoding exo and bet. This original combination of mutants were named and saved as the strain indicated as the donor in this cross.

i

This deletion allele was created by first transducing the kan resistant derivative from the Keio collection into the strain as indicated in the reference column. pLH29, carrying the flp gene, was then introduced and Kan sensitive derivatives were screened.

j

recX::cat was amplified with prSJS748,749 using pACYC184 (New England Biolabs) as a template. recX::cat was transferred to the chromosome using the exo-bet method next to the recA allele indicated. This original combination of mutants were named and saved as the strain indicated as the donor in this cross.