Skip to main content
. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Hear Res. 2009 Apr 5;254(1-2):1–14. doi: 10.1016/j.heares.2009.03.014

Table 2.

Antibodies: source, type, dilution, specificity, immunogen, and controls.

Antibody/
Source/ Cat #		 Antibody type/
dilution		 Specificity/
Immunogen		 Positive/
negative controls
Collagen IVα2/
Chemicon
Int./MAB1910		 Mouse monoclonal
IgG
/ 1:500		 Human type IV collagen α2 chains/
Human amniotic type IV collagen		 Human kidney and brain BMs
(Fig 9)/
Omission of the antibody (no
reaction)
Nidogen-1/
Calbiochem/
481918		 Rabbit polyclonal IgG
/ 1:500		 Human nidogen/ Purified, human
placental nidogen.		 Human kidney and brain BMs
(Fig 9)/
Omission of the antibody (no
reaction)
Tenascin-C/
Chemicon Int./
AB19013		 Rabbit polyclonal IgG
/ 1:1000		 Tenascin chicken, human and
mouse/
Purified tenascin from embryonic
chicken brain		 Mouse cochlea (Fig 9)/
Omission of the antibody (no
reaction)
Laminin-β2/
Upstate/
05–206		 Rat monoclonal IgG
/ 1:200		 Human and mouse β2 chain laminin/
Murine EHS laminin		 Human kidney BMs(Fig 9)/
Omission of the antibody (no
reaction)
αDystroglycan
Upstate/
05–593		 Mouse ascites IgM
/ 1:250		 αDystroglycan from human, mouse,
rat/ rabbit skeletal muscle membrane
preparation		 Human kidney and brain BMs
(Fig 9)/
Omission of the antibody (no
reaction)
Aquaporin-4/
Santa Cruz/
Sc-20812		 Rabbit polyclonal IgG
/ 1:1000		 AQP4 mouse, rat and human/ amino
acids 244–323 mapping at the C-
terminus of AQP4 of human origin		 Human cochlea and brain (Fig 9)/
Omission of the antibody (no
reaction)

Chemicon Int. (Temecula, CA); Upstate (Temecula, CA); Calbiochem (La Jolla, CA); Santa Cruz Biotechnology (Santa Cruz, CA)