Fig. 1.

SWCNT induces ROS in BJ Foreskin Cells. 10,000 cells/well was seeded in a 96 well plate and incubated for 18 h. Cells were then washed with Hank's Balanced Salt Solution (HBSS) followed by incubation in CO₂ incubator with 10 μM DCF for 3 h in HBSS. The cells were then washed with HBSS and incubated with different concentrations of SWCNT and 100 μM H₂O₂ in DMEN containing 10% FBS. Fluorescence was read at the end of 3 h. The fluorescence values were normalized with the protein content in each well. Values are mean±SD of 8 wells and is a representative from three experiments performed independently.