Fig. 5.

Relative RT-PCR analysis of ATM, CCNC, DNAJB4, and GADD45A expression in BJ Foreskin cells treated with SWCNT. (A) BJ Foreskin cells were treated with SWCNT as detailed in text for 24 h. Total RNA was isolated and DNase treated before RT. Relative RT-PCR was performed as described in text and c-DNA was amplified using specific primers for indicated genes. PCR products were separated on 1% agarose gel and stained with ethidium bromide as shown in representative image. GAPD was used as internal control. (B) By densitometry analysis, ATM, CCNC, DNAJB4, and GADD45A gene expression was evident in 24 h. The band intensity of GAPD was used to normalize expression of genes.