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Titration of variants and Vitaxin Fab on immobilized αvβ3. Bacterial cell lysates containing Vitaxin (•), variants with improved affinity isolated from the primary libraries (S102, ▪; Y100, □; and Y101, ▵) or from the combinatorial libraries (clone 17, ▴), or an irrelevant Fab (○) were titrated on immobilized αvβ3 as described in Materials and Methods.
