Figure 2.

(A) Inhibition of fibrinogen binding to immobilized α_vβ₃. Immobilized α_vβ₃ was incubated with 0.1 μg/ml of biotinylated fibrinogen and various concentrations of Vitaxin (○), S102 (•), F32 (▵), or C59 (▴) for 3 h at 37°C. Unbound ligand and Fab were removed by washing, and bound fibrinogen was quantitated after incubation with NeutrAvidin alkaline phosphatase conjugate. (B) Correlation of affinity of variants with inhibition of fibrinogen binding. The concentration of variants required to inhibit the binding of fibrinogen to immobilized α_vβ₃ by 50% (IC₅₀) was plotted as a function of the affinity (K_d).