Table 1.

Capture lift screening of Vitaxin CDR libraries

Library	Size*	Screened†	Positives‡	Enhanced affinity§
H1	320	2,500	16	8
H2a	320	5,000	26	7
H2b	320	5,000	2	1
H3	320	2,500	98	78
L1	416	2,500	12	1
L2	352	3,250	7	1
L3	288	5,000	52	41

^*Number of unique clones based on DNA sequence. Thirty-two codons are used to encode all 20 amino acids at each position. 

^†Phage-expressed libraries were plated on XL-1 blue/agar lawns at 500-1,000 plaques per 100-mm dish. 

^‡Positives are defined as clones that were identified in the initial screen, replated, and verified in a second capture lift assay. 

^§Soluble Fab was titrated against immobilized α_vβ₃ in an ELISA format. Based on comparison of the inflection point of the titration profiles, clones that displayed ≈3-fold enhanced affinity were selected for further characterization.