Table 3.

Identification of optimal combinatorial mutations

Library	Clone	Sequence
		L1 32	L3 92	L3 96	H2 60	H3 97	H3 101	H3 102	kon (×104), M−1 s−1	koff (×10−3), s−1	Kd, nM
Wild type		H	G	H	L	Y	A	Y	18.0	4.97	27.6
F32	17	F						S	25.1	0.138	0.5
	7	F			P	H		S	20.4	0.236	1.2
	56	F			P			S	26.6	0.135	0.5
	C59	F			P			D	26.5	0.137	0.5
	C176	F			P			T	22.5	0.192	0.9
	V357D	F						D	27.9	0.140	0.5
N92	C119		N		P			S	21.5	0.316	1.5
L96	8F9			L	P	H		S	47.5	0.280	0.6
	C29			L	P	H	Y	S	67.5	0.343	0.5
	2G4			L				S	60.3	0.229	0.4
	C37			L			Y	E	44.8	0.147	0.3
	6D1			L	P		Y	S	41.0	0.158	0.4
	6G1			L	P			S	38.9	0.280	0.7
	6H6			L		H		S	50.4	0.187	0.4