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. Author manuscript; available in PMC: 2009 Oct 6.
Published in final edited form as: Biochemistry. 2008 Jun 3;47(26):6883–6894. doi: 10.1021/bi800519a

FIGURE 5.

FIGURE 5

MB rescue of CUCG3′SA cleavage for wild-type and modified ribozymes. Rescue reactions were carried out as indicated in the text and in Materials and Methods. (A) The metal ion-substrate contact being probed is indicated by green solid dots in the model for the Tetrahymena ribozyme transition state. X represents an oxygen or sulfur atom in the guanosine analogues CUCGA and CUCG3′SA, respectively. (B) Rescue profiles for the wt (○), C208SP (red △), A304SP (blue △), A306SP (gray △), and U307SP (orange △) ribozymes. krel represents the observed rate constant for cleavage of CUCG3′SA relative to CUCGA and was normalized to equal 1 in the absence of Cd2+.