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. Author manuscript; available in PMC: 2009 Oct 6.
Published in final edited form as: Biochemistry. 2008 Jun 3;47(26):6883–6894. doi: 10.1021/bi800519a

Table 4.

Observed Rate Constants for the Reaction (E•S3′O • xG)o → Products (kopen; 50 mM MgCl2, pH 6.9) with Different Ribozymes without and with 1 mM CdCl2 a

ribozyme Mgkopen
(min−1) × 10−2 		fold down
relative to wt		 Cdkopen
(min−1) × 10−2 		Cdkopen/
Mgkopen
wt 2.6 (1) 1.8 0.69
C208SP 0.046 57 0.069 1.5
C208RP 0.066 39 0.057 0.86
A304SP 0.018 140 0.032 1.8
A304RP 0.44 6 1.1 2.5
A306SP 0.042 62 0.058 1.4
A306RP 0.0095 270 0.0058 0.61
U307SP 0.26 10 0.27 1.0
U307RP 0.25 10 2.0 8.0
a

S3′O is defined in the text (xG = G or UCG).