Fig. 5.

Luciferase reporter gene transfer efficiency of the polymer/pDNA polyplexes and metabolic activity of the transfected CHO-K1 and B16-F10 cells. All the polyplexes were prepared in HBG buffer; 200 ng of pDNA/well was used for 96-well tissue culture plate. The cell culture medium was changed 4 h after polyplexes addition; the relative luciferase intensity (a, c) and metabolic activity of the polyplexes treated cells (b, d) was measured 24 h later. Presented data show the average of experiments performed three times as triplicates (n = 3 × 3)