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Free steroids; specific ions monitored for GC-EI-MS/MS
| Compound | MRM transition 1 (m/z) | Collision energy MRM transition 1 (eV) | MRM transition 2 (m/z) | Collision energy MRM transition 2 |
|---|---|---|---|---|
| 17α-estradiol | 416 > 326 | −7.5 | 416 > 285 | −10.0 |
| 17β-estradiol | 416 > 326 | −7.5 | 416 > 285 | −10.0 |
| 17β-estradiol-d3 | 419 > 285 | −10.0 | ||
| 17α-testosterone | 432 > 327 | −15.0 | 432 > 209 | −12.5 |
| 17β-testosterone | 432 > 301 | −15.0 | 432 > 209 | −12.5 |
| 17β-testosterone-d2 | 434 > 211 | −12.5 |
