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. 2009 Jan 3;281(3):329–344. doi: 10.1007/s00438-008-0410-x

Fig. 2.

Fig. 2

Transcripts of the Dart1 transposase genes detected in rice and transgenic Arabidopsis plants. a Structure of Dart1-27 in the pyl-v genome. The large horizontal filled arrowheads and the shadowed areas at both ends of the transposon Dart1-27 box indicate the terminal inverted repeats and subterminal regions, respectively. The transposase gene carries an approximately 92-bp intron in its 5′-untranslared region, and its mRNA is indicated under the Dart1-27 box; the open pentagonal arrow in the 2nd exon of the mRNA boxes represents the coding region of the transposase gene. Probe A was used for screening Fosmid clones in Fig. 1, and the horizontal arrowheads with the codes (numerals with either F or R) represent primers for PCR or RT-PCR amplification and RACE analysis (Supplementary Table S1). b Schematic representation of the alternatively spliced transposase transcripts observed in rice and transgenic Arabidopsis plants. Three different transcripts in sizes (L, M, and S) were observed in rice, and their splicing patterns are categorized. The premature stop codons are indicated in parentheses, and the sizes of the RT-PCR amplified fragments were indicated in brackets. For detailed structural information concerning the individual transcript, see Supplementary Table S2. c Transcripts of the Dart1 transposase genes in rice by RT-PCR analysis. Lane 1, pyl-v; lane 2, pyl-stb; lane 3, Nipponbare; lanes 4–6, three different pyl-stb plants carrying a single copy of Dart1-27* generated by transforming pZEN12; lanes 7–9, three different pyl-stb plants carrying a single copy of Dart1-27D generated by transforming pZEN13. OsUbi (Os03g0234200) was used as an internal control. d Transcripts of the Dart1 transposase genes in Arabidopsis by RT-PCR analysis. Vectors used for transformation are lane 1, pZEN2; lane 2, pZEN3; lane 3, pZEN4; lane 4, pZEN5; lane 5, pZEN6; lane 6, pZEN7; lane 7, pZEN8; lane 8, pZEN9; lane 9, pZEN10. ATTUB4 (M21415) was used as an internal control. The numerals in parentheses indicate the cycles of PCR amplification