Fig. 5.

Excisions of nDart1-0 and Dart1-27 from the introduced vectors and generations of footprints upon excisions of the elements in transgenic Arabidopsis plants. a Excisions of nDart1-0. The filled and open arrowheads point to the PCR-amplified bands with and without the nDart1-0 insert, respectively, in the PCR analysis. The target site duplication (TSD) sequences of nDart1-0 originated from the pyl-stb plant are underlined. b Excisions of Dart1-27 and other Dart1 elements examined. The open arrowheads point to the PCR-amplified bands without the Dart1 inserts in the PCR analysis. The TSD sequences of Dart1-27 originated from Nipponbare are underlined. The numerals in the fractional expressions in the parentheses at the rightmost column indicate the occurrences of the footprint sequences observed. Footprints generated by the excisions of other Dart1 elements examined are shown in Supplementary Fig. S1