Fig. 7.

mRNA expression of selected Kv channels, accessory sub-units and modulating factors of Kv4.2. a Relative ratios of mRNA for EPI and ENDO samples from SED and TRN hearts. a Kv4.2 expression was dependent upon region, being significantly greater in EPI than ENDO (*P < 0.05 two-way ANOVA). Kv4.3 and Kv1.4 expression was not different between EPI and ENDO. Exercise training had no significant effect upon expression levels. b KChIP2 mRNA expression was significantly greater in EPI than ENDO, a significant but reversed transmural gradient was seen for Irx-5 (*P < 0.05, two-way ANOVA). There was no transmural gradient for calcineurin A beta. Voluntary exercise did not significantly alter the expression levels of any of the three targets (P > 0.05, two-way ANOVA). Expression of all mRNA levels is relative to GAPDH (n = 10 in all cases)