Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Gene specific primer pairs used for RT-PCR
| Target Gene | Kv1.4 | KChIP2 |
|---|---|---|
| GenBank accession number | NM_012971 | BC085905 |
| Forward primer sequence (5′–3′) | TGGGTGTTCCTACACTGACCTACT | ACCACGATGGCTCTGTCAGTTTT |
| Reverse primer sequence (5′–3′) | GAAGCGTAGACCAGACACATT | TCACCACGCCGTCCTTGTTC |
| Annealing temperature (°C) | 56 | 56 |
Primers were designed using the published GenBank sequences for the rat genes listed
