Table 2.
Contractile parameters of skinned fibers before mcTnC extraction and after reconstitution with hcTnCL29Q or hcTnCWT and incubation with phosphatase
| Passive force (%) | FMAX (%) | pCa50 | nH | |
|---|---|---|---|---|
| L29Q, before mcTnC extraction | 2.8 ± 0.6 | 100 | 5.48 ± 0.02 | 5.45 ± 0.02 |
| L29Q, after reconstitution, PP1c | 4.2 ± 0.7** | 69 ± 3*** | 5.55 ± 0.03 | 4.16 ± 0.37* |
| WT, before mcTnC extraction | 3.4 ± 1.0 | 100 | 5.47 ± 0.02 | 5.56 ± 0.78 |
| WT, after reconstitution, PP1c | 4.0 ± 0.6 | 66 ± 1*** | 5.50 ± 0.03 | 4.59 ± 0.27 |
Data are given as mean ± SEM, n = 5, passive force was measured at pCa 8.0 and FMAX (maximal force) at pCa 4.6. Passive force and FMAX were normalized to FMAX of the first force–pCa relation
* P < 0.05, ** P < 0.01, *** P < 0.001