Table 4.
ΔpCa50 | WT | L29Q |
---|---|---|
Before/after cTnC-exchange | +0.06 ± 0.044 | +0.06 ± 0.030 |
Before/after cTnC-exchange + PPlc | −0.04 ± 0.030 | −0.08 ± 0.030 |
PPlc, before/after PKA | +0.18 ± 0.004 | +0.20 ± 0.022 |
Table 4 summarizes the changes in pCa50 induced in skinned fibers (1.) by exchange with the recombinant hcTnCs (WT/L29Q), (2.) by exchange with the recombinant cTnCs and additional treatment with phosphatase, and (3.) the ΔpCa50 induced by incubation of the phosphatase-treated, exchanged fibers with PKA