Table 1.

Effects of α_6EX antibody on neurite formation and neurite outgrowth by retinal neurons

Substrate	IgG	% cells with neurites	Average neurite length
LN	−	70	30.1±2.2
LN	α6EX	32	25.6±2.1
LN	NR	71	31.4±1.6
col IV	−	55	26.6±1.9
col IV	α6EX	55	26.8±1.9
col IV	NR	52	24.7±1.9

Dissociated E6 retinal neurons were plated on laminin (LN)- or collagen IV (col IV)-coated substrata and incubated for 24 hours at 37°C in culture medium plus no IgG (−), 0.5 mg/ml of α_6EX IgG (α_6EX) or 0.5 mg/ml of normal rabbit IgG (NR), as described in Materials and Methods. For quantitation of neurite formation by attached cells, 200 neurons in each culture condition were scored for the presence or absence of neurites. Results are presented as % of cells with neurons. For quantitation of neurite length, 50 neurons with neurites were examined in each culture condition. Neurite length is indicated as average neurite length ± standard error.