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. Author manuscript; available in PMC: 2009 Oct 6.
Published in final edited form as: Development. 1993 Jun;118(2):377–388. doi: 10.1242/dev.118.2.377

Table 2.

Effect of tectal ablations on α6 mRNA levels in retinal ganglion cells (RGC) and cells depleted of retinal ganglion cells (non-RGC)

α6 mRNA ablation
α6 mRNA control
Experiment RGC Non-RGC
1 1.45 0.99
2 0.76 1.29
3 1.15 0.89
4 1.47 0.84
average 1.21±0.33 1.00±0.20

Retinal ganglion cells were separated from other cells on Percoll gradients as described in Materials and Methods. Each experiment used 3–5 embryos for each sample. The complete optic tecta were removed from embryos in windowed eggs at E5-6. Embryos were sacrificed between E9-11; total RNA was prepared and specific transcripts were quantitated by RNA blot as described in Materials and Methods. Values were normalized using β-actin mRNA as a control. Values in the table are ratios of normalized α6 mRNA levels in ablated versus control animals. Average values ± standard deviations are indicated.