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ISL1 and BRN3B regulate the expression of a common set of RGC-specific genes. (A–H) Compared with controls (left panels), in situ hybridization shows that at E14.5, the expression of RGC specific genes Brn3a, Isl2, Olf1, Irx4, Ablim1, Gap43, L1cam, and Shh decreases in the GCL (bracket) of both Isl1-null (middle panels) and Brn3b–null retinas (right panels). GCL, ganglion cell layer; L, lens; T, temporal; N, nasal. Scale bar: 100 μm.
