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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9556–9568. doi: 10.1021/bi901110n

Table 1.

Fe and protein concentrations in packed mitochondria, and activities in isolated mitochondrial suspensions (the error for activity assays is estimated at 20%).

W303/Glu/O2 Gal- ATM1/Glu/O2 W303/Glu/Ar Gal- ATM1/Glu/Ar
[Fe] (μM) 630 ± 40 8900 ± 400 170 ± 10 180 ± 10
[Protein] (mg/mL) 57 ± 5 26 ± 3 31 ± 4 34 ± 5
Aconitase activity (units/mg protein) 670 80 650 700
Succinate dehydrogenase activity (units/mg protein) 14 5 (not determined) (not determined)
Leu1p activity (units/mg protein) 150 N.D.a 320 N.D.a
a

N.D., activity not detectable (lower than 20 units/mg protein).