Table 1.
Fe and protein concentrations in packed mitochondria, and activities in isolated mitochondrial suspensions (the error for activity assays is estimated at 20%).
| W303/Glu/O2 | Gal- ATM1/Glu/O2 | W303/Glu/Ar | Gal- ATM1/Glu/Ar | |
|---|---|---|---|---|
| [Fe] (μM) | 630 ± 40 | 8900 ± 400 | 170 ± 10 | 180 ± 10 |
| [Protein] (mg/mL) | 57 ± 5 | 26 ± 3 | 31 ± 4 | 34 ± 5 |
| Aconitase activity (units/mg protein) | 670 | 80 | 650 | 700 |
| Succinate dehydrogenase activity (units/mg protein) | 14 | 5 | (not determined) | (not determined) |
| Leu1p activity (units/mg protein) | 150 | N.D.a | 320 | N.D.a |
N.D., activity not detectable (lower than 20 units/mg protein).