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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9327–9329. doi: 10.1021/bi901354z

Figure 1.

Figure 1

Pyrene-actin polymerization curves of 4 μM actin (5% pyrene labeled) with 2.5 nM mDia1-FH2 and the indicated μM concentrations of 1 (A) or 2 (B). (C) Dose-response curves for 1 and 2 on mDia1-FH2 activity, calculated from pyrene-actin curves. (D) Effects of compounds (50 μM) on polymerization of 4 μM actin (5% pyrene labeled). Compound 2 curve was normalized by a factor of 1.75, after subtracting fluorescence background. (E) Pyrene-actin assay with 2.5 nM mDia3-FH2 with or without 2 (50 μM). (F) Dose-response curves for 2.3 on pyrene-actin polymerization in the presence of 2.5 nM mDia2-FH2, 20 nM INF2-(FH1-FH2), 200 nM FRL1-(FH1-FH2), or 10 nM mDia3-FH2.