Figure 2.

Formation of ΔΨ by wild-type Na⁺-NQR and acidic mutants reconstituted into proteoliposomes. A) Formation of ΔΨ by wild-type Na⁺-NQR showing the effects of CCCP (5µM), ETH-157 (5µM) and the omission of NaCl from the medium. B) Formation of ΔΨ by wild-type Na⁺-NQR and acidic mutants. The assay buffer contained: 100 µM Q-1, 100 mM HEPES, 100 mM NaCl (except where noted), 150 mM KCl, 1 mM EDTA, pH 7.5. The reaction was started by the addition of NADH (200 µM).