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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9516–9524. doi: 10.1021/bi900845y

Table 1.

Primers designed for the site directed mutagenesis used in this work.

Subunit Mutation Primer sequence (5’→3’)
B
E28A GTGGTTTGCCCTGTATGCCGCGGCGGCGACGCTG
E144L CTATCTACGCTACGGTGTTCATCGTCGGTGGTTTCTGGCTCGTGTTG
TTCTGTATGGTGC GCAAGCATGAAGTCAAC
D223A GCACAGATCTCAGGAGCTCTAGTATGGACTGCGG
E274A GGTAACATCCCAGGTTCAATTGGCGCCGTGTCTACTCTGGCACTCA
TGATTG
D346A GGTATGTTCTTCATGGCGACGGCGCCATGGTCTGCGTCCTTCACC
E380A GTGAACCCGGCTTACCCAGCCGGCATGATGCTGGCGATC
D397A GCGAACCTATTTGCGCCACTGTTTGCGCATGTGGTTGTAGAGAAAT
ATCAAG
D
D17A GAGTGTGTTAGCCCCAGTGCTAGCCAACAACCCGATTGCACTGC
E39L GCGGTAACCACTAAGCTGTTAACGGCATTTGTTATGACGCTA
D87L TCGCTTCGTTAGTTATCGTGGTACTGCAGATTCTGAAAGCGTATCTG
E119A CGTAATGGGTCGTGCCGCGGCGTTTGCAATGAAGTCTG
D133A TCTGAACCAATTCCTTCTTTCATTGCCGGCATTGGTAACGGTTTAGG
A
E153A CTGATGACGGTTGGTTTCTTCCGAGCTCTTTTAGGCTCAGGTAAG
E
E15A CTGCTGGTGAAATCGATTTTCATCGCTAACATGGCACTAAGCTTCTT
CCTAGGG
E95A GCGGCATTATGACAGATTCTGGCTATGATCCTCGATCGC
E138A CAGCGCGACTACAGCTTTGCTGCTAGCGTGGTATACGGTTTCGGTT
C
E162G GTCGCTCTTGCAGGTATCCGCGGTAAGATGAAGTATTCAGAC

The underlined bases indicate the sites that were modified to introduce the mutations