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Binding titrations for propyl gallate (a) and 4-hexylresorcinol (c) and transactivation assays for propyl gallate (b), 4-hexylresorcinol (d), and 17β-estradiol (e). The antagonist activity of propyl gallate was tested in a transactivation assay (f). Each experiment was carried out in triplicate, according to the procedure reported in the Experimental Procedures.
