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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2008 Nov 27;239(2):130–136. doi: 10.1016/j.taap.2008.11.011

Figure 2. MMAIII treatment of WI-38 cells leads to increased synthesis of TrxR1 at the expense of other selenoproteins.

Figure 2

WI-38 cells were treated with MMAIII at the concentrations indicated in triplicate. During exposure to MMAIII, 75Se (selenite) was added to the culture medium and cells were incubated for 24 hours. Crude cell extracts (25 μg protein) were resolved by SDS-PAGE (15%) and labeled selenoproteins were visualized using phosphor screen. TrxR1 – cytosolic thioredoxin reductase; Arrow labeled with an asterisk indicates a selenoprotein used for analysis of selenium incorporation into proteins other than TrxR1.