Foxp3 F	5′-GAGAAGCTGAGTGCCATGCA-3′ and
FoxP3rev	5′-AGGAGCCCTTGTCGGATGAT-3′
probe: 5′-FAM-ATGGCACTGACCAAGGCTTCATCTGTG-eclipse dark-3′;
β-actin primers,	5′-CCTGGCACCCAGCACAAT-3′
and	5′-GCCGATCCACACGGAGTACT-3′ and the
β-actin probe, 5′-VIC-ATCAAGATCATTGCTCCTCCTGAGCGC-Yahima yellow-3′.