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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Hepatology. 2009 Sep;50(3):834–843. doi: 10.1002/hep.23052

Figure 4. COX-2-deficiency enhances Fas-induced hepatocyte apoptosis and liver tissue damage.

Figure 4

The COX-2 knockout (KO) mice and their age/sex-matched wild type mice (6 mice per group) were administered intraperitoneally with saline or Jo2 (0.5 µg/g body weight). The animals were sacrificed at 0 (a and b) and 4 hours (c and d) after Jo2 injection and the liver tissues were harvested for histological evaluation. Formalin-fixed and paraffin-embedded sections (5 µm thick) were stained with hematoxylin and eosin (H&E) (A), and terminal deoxynucleotidy1 – transferase-mediated deoxyuridine triphosphate-digoxigenin nick-end labeling (TUNEL) (B) (200X). The livers of COX-2 knockout mice exhibit more prominent hepatocyte apoptosis and hemorrhage (d) when compared to the livers of wild type mice (c). The number of TUNEL-positive hepatocytes in COX-2 knockout mice is significantly higher than in wild type mice (*p<0.01; the data are expressed as mean ±SD from 6 mice) (C).