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. Author manuscript; available in PMC: 2009 Oct 7.
Published in final edited form as: Cell. 2008 Aug 8;134(3):451–460. doi: 10.1016/j.cell.2008.06.028

Figure 3. Sestrin inhibit p70S6K phosphorylation via an AMPK-dependent mechanism.

Figure 3

(A) Sesn2 expression enhances AMPK phosphorylation. Sesn2F was induced in MCF7-tet OFF Sesn2F cells and phosphorylation of the indicated proteins was examined 24 hrs later. (B) Compound C abrogates Sestrin-induced inhibition of p70S6K phosphorylation. Sesn2F was induced in MCF7-tet OFF Sesn2F cells in the presence of compound C (CompC) or vehicle control (DMSO) and protein phosphorylation was examined 24 hrs later. (C) Knock-down of AMPKα1 abrogates Sesn2 inhibition of p70S6K and S6 phosphorylation. MCF7-tet OFF Sesn2F cells were infected with shAMPKα1- or shLuc-lentiviruses. Protein phosphorylation and expression were analyzed by immunoblotting 72 hrs after infection and 24 hrs after doxycycline removal.