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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Methods Mol Biol. 2009;557:65–76. doi: 10.1007/978-1-59745-527-5_6

Fig. 1.

Fig. 1

Asci from h90 meiosis.

Cells of an h90 strain were washed and spotted on a supplemented SPA plate, which was incubated for 3 d at 25°. A sample of the cell-ascus mixture was observed under a differential interference contrast microscope and photographed. (Left) The linear ascus (A) has the appearance of an azygotic ascus, whereas the curved ascus (B) has that of a zygotic ascus. A zygote (Z), an unmated cell (C), and a spore (S) from spontaneous lysis of an ascus are also visible in this rare field. (Right) A more typical field of asci, one of which (Z) is immature.