Fig. 2.

Assembly of Petri dish with acetone-soaked paper for killing vegetative cells.

A replicating velveteen (a) is stretched over the elevated plastic block (b) and held in place with the clear plastic ring (c). A master plate with colonies is replicated onto the velveteen. Acetone is pipetted onto the filter paper (d) held in the glass Petri dish (e) with an internal plastic ring (f). This assembly is then inverted over the velveteen. After exposure to the acetone vapors, the cell-ascus mixtures on the velveteen are replicated onto a fresh YEA plate.