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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Immunol Lett. 2009 Jun 23;125(2):114–118. doi: 10.1016/j.imlet.2009.06.007

Figure 1. Dendritic cells differentiated in the presence of IL-10 (Tol-DCs) display an impaired ability to present cognate antigen to antigen-specific CD4+ T-cells.

Figure 1

BM-DCs and Tol-DCs were generated from the bone marrow of BALB/c mice. The expression levels of CD11c (A), B7.2 (B) and MHC class II molecules (C) were determined by flow cytometric analysis. Numbers in A (right and middle panel) represent the percent of CD11c positive DCs. % of Max is the intensity of each curve relative to the 100% value of the histogram with the highest number of analyzed cells as assigned by the Flow-Jo software. Shown is a representative experiment of three independent experiments with similar results. In parallel experiments, APCs were evaluated for their ability to stimulate cognate T cell responses, as described in Materials & Methods. Functional capacity of naïve anti-HA CD4+ T cells (CD4+ CD62L+) from TCR transgenic HA +/- mice were determined based on HA-specific proliferation (D) and IFN-γ production (E). Data represent mean ± s.d. of triplicate cultures. Shown is a representative experiment of two independent experiments with similar results.