Figure 4.
Mutation of Thr34 to Ala in DARPP-32 prevents haloperidol-induced histone H3 phosphorylation. Wild type (WT) or T34A DARPP-32 mutant mice were treated with vehicle or haloperidol and perfused 15 min later. (a) Phospho-Ser10-acetyl-Lys14-histone H3 (P-AcH3) immunoreactivity in single confocal sections of the dorsal striatum from WT or T34A mutant mice. (b) Quantification of P-AcH3 immunoreactive neurons in the dorsomedial (DM) and dorsolateral (DL) striatum, 15 min after administration of vehicle (Veh) or haloperidol (Hal) to WT or T34A DARPP-32 mutant mice (T34A) (* p < 0.05, *** p < 0.001 vs. WT Veh; °°p < 0.01, °°°p < 0.001 vs. WT Hal). Scale bar: 40 µm.