Figure 6.
Expression of MSK1 in striatonigral and striatopallidal MSNs. (a) MSK1 immunoreactive nuclei (red) are co-labeled with EGFP (green) in the striatum of Drd1a- or Drd2-EGFP mice in a double fluorescence analysis. Nuclei intensely labeled with MSK1 antibodies colocalized with EGFP in Drd1a-EGFP but not in Drd2-EGFP mice (arrowheads). Single confocal sections, scale bar: 40 µm. (b) Quantitative analysis of MSK1 fluorescence intensity in Drd1a- and Drd2-EGFP mice. Distribution histograms of intensely labeled MSK1 nuclei (average normalized immunofluorescence intensity > 120 a.u.) colocalized with EGFP in Drd1a-EGFP mice (black bars) and in Drd2-EGFP mice (white bars). (c) Mean values of MSK1 immunofluorescence (arbitrary units, a.u.) of neurons containing (EGFP+) or not (EGFP-) EGFP in Drd1a- and Drd2-EGFP mice (*** p < 0.0001).