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. Author manuscript; available in PMC: 2009 Oct 7.
Published in final edited form as: Neuron. 2003 Oct 30;40(3):501–514. doi: 10.1016/s0896-6273(03)00666-4

Figure 7. Adenoviral Recombination of Meningeal Fibroblasts Is Sufficient to Induce Cortical Dysplasia.

Figure 7

(A and B) Paraffin sections (7 µm) from E18.5 embryo heterozygous (+/flox, [A]) or homozygous (flox/flox, [B]) cortex following in utero injection of an adenovirus bearing Cre recombinase and GFP at E12.5/13 and stained with DAPI (blue) and anti-GFP (green). Cortical ectopias were present in the marginal zone of the flox/flox animal and coincided with interrupted laminin staining of the cortical basement membrane (inset, [B]).

(E–H) Laminin staining of primary meningeal fibroblasts isolated from E15 (flox/flox) and infected with either an adenovirus expressing GFP alone (E and G) or GFP plus Cre recombinase (F and H). fak−/− fibroblasts were unable to organize laminin into fibrillar structures as in control. Scale bar, 50 µm (B) and 25 µm (A–D).