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. Author manuscript; available in PMC: 2009 Oct 7.
Published in final edited form as: Am J Physiol Renal Physiol. 2007 Aug 1;293(4):F1272–F1281. doi: 10.1152/ajprenal.00138.2007

Fig. 6.

Fig. 6

Impact of aging on MMP-7 expression. A: MMP-7 gene expression was assessed using quantitative PCR; the β-actin-normalized MMP-7 gene expression is presented relative to the gene expression in an arbitrary reference sample (Stratagene Rat Universal Reference RNA). The values represent means ± SD of relative gene expression of 4 animals per group. *Significant difference in 24-mo AL compared with young. **Significant difference in aged-AL compared with young and aged-CR. B: Western blot analysis of MMP-7 protein levels in kidney lysates. Full-length MMP-7 is seen at ~27 kDa; membranes were stripped and reprobed with an antibody against β-actin to demonstrate equal loading. C: paraffin-embedded sections were processed for immunohistochemical localization of MMP-7 with a commercially available system; similar results were seen in duplicate experiments.