Figure 4. The processing of fem and Am-dsx transcripts in the response to the knockdowns of the csd and the fem gene induced by RNAi.

(A) The male and female fem and Am-dsx mRNAs of eight 5^th larval instar pseudomales that have been injected with csd siRNAs. Fragments corresponding to the fem female (∼350 bp) and male (∼1.6 kb) mRNAs and the Am-dsx female (∼1.4 kb) and male (∼500 bp) mRNAs were amplified by RT-PCR and resolved by agarose gel electrophoresis. The fragments obtained from untreated females and males are shown in the 1^st and 2^nd lane, respectively. (B) Same analysis as in (A) except that eight pseudomales have been treated with fem instead of csd siRNAs.