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. 2009 Oct 12;4(10):e7422. doi: 10.1371/journal.pone.0007422

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Masson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Staining of neutral lipids. Plates of 3T3F442A transfected with Luc siRNA (panel a) or LRP1 siRNA1 (panel b) at day 7 are presented. Two-days post Luc or LRP1 siRNA transfection, confluent 3T3F442A cells were grown in the presence of the adipogenic differentiation medium. After 7 days of differentiation, the extent of cellular lipid accumulation was revealed by oil Red O staining. A representative experiment out of 3 is shown. (B) Micrographs of 3T3F442A preadipocytes. Cells were transfected with Luc siRNA (panels a and c) or LRP1 siRNA1 (panels b and d) and micrographs were performed after 3 (panels a and b) and 7 (panels c and d) days of differentiation. A representative experiment out of 3 is shown. (C) Quantification of neutral lipids and triglycerides. In panel a, lipid content was quantified at 3, 5 and 7 days of differentiation in 3T3F442A transfected with Luc or LRP1 siRNAs. Mean ± SD of 3 independent experiments is shown. *P<0.0025 when compared with Luc siRNA transfected cells. In panel b, triglycerides were quantified at day 5 and 7 of differentiation in 3T3F442A transfected with Luc or LRP1 siRNAs. Results were normalised per µg of DNA. Data are presented as mean ± SD of an experiment performed in triplicate. *P<0.0025 when compared with Luc siRNA transfected cells.