Skip to main content
. 2009 Oct 12;4(10):e7422. doi: 10.1371/journal.pone.0007422

Figure 8. LRP1 silencing in fully-differentiated adipocytes leads to lipid-depleted cells.

Figure 8

(A) Silencing of LRP1 in fully-differentiated adipocyte. 3T3F442A pre-adipocytes were maintained for 10 days in adipogenic differentiation medium (panel a) and were transfected with Luc siRNA or LRP1 siRNA1. LRP1β protein expression was monitored by immunoblotting 2 days post-transfection (panel b). ERK2 was used as a loading control. (B) Staining of neutral lipids. Plates of differentiated 3T3F442A transfected with Luc siRNA (panel a) or LRP1 siRNA1 (panel b) are shown. After siRNA transfection, 3T3F442A mature adipocytes were grown in DMEM + 10% FCS. The extent of cellular lipid accumulation was determined at day 2 and 7 of culture by oil Red O staining. A representative experiment out of 3 is shown. (C) Micrographs of 3T3F442A adipocytes. Micrographs of differentiated adipocytes transfected with Luc siRNA (panel a) or LRP1 siRNA1 (panel b) were performed 7 days post-transfection. A representative experiment out of 3 is shown. (D) Quantification of lipid content. Lipid content was quantified at days 2 and 7 post-tranfection of differentiated adipocytes transfected with Luc or LRP1 siRNA1. Mean ± SD of 3 independent experiments is shown. *P<0.005 when compared with Luc siRNA transfected cells.