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. 2009 Oct 15;4(10):e7469. doi: 10.1371/journal.pone.0007469

Figure 3. Contribution of AU-rich elements to IL-3 control of transcript stability.

Figure 3

A. Enrichment for ARED sequences in IL-3-stabilized transcripts. The excess accumulation of AU-rich transcripts as a function of position in the ranked list is shown (blue). An upward slope indicates that AU-rich sequences are accumulating at a faster rate than predicted by random ranking; a downward slope indicates that ARE's are accumulating at a slower rate than random transcript ranking would predict. The 95th percentile confidence interval curve for random rankings is also plotted for comparison (red). B. IL-3 response element mapping in the IL6 promoter. A 158-bp IL6-3′-UTR fragment destabilizes a heterologous transcript in the absence, but not in the presence, of IL-3. Northern blotting of RNA from 32Dcl3 cells stably transfected with EGFP or an EGFP-IL6UTR transgene. GAPDH indicates loading and pik3cd demonstrates endogenous transcript regulation in the presence and absence of IL-3. Representative blots of two independent experiments are shown.