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. 2009 Oct 14;4(10):e7462. doi: 10.1371/journal.pone.0007462

Figure 2. Cellular locations of MelD2 and MelC2.

Figure 2

Samples of 10 ml of Streptomyces cultures grown to late log phase in TSB were harvested. Cytosolic (C), membrane (M), and extracellular (E) fractions of the cultures were fractionated and the proteins separated by SDS gel electrophoresis. MelD2-His and MelC2 proteins were detected by immunoblotting using anti-polyhistidine (A) and anti-MelC2 (B) antibodies, respectively. B19 is a ΔmelC mutant of S. avermitilis isolated in this study. YU5 is a ΔmelC mutant of S. antibioticus isolated previously [10]. M145/pIJ702-117 is M145 harbouring pIJ702-117, a high-copy-number plasmid containing the melC operon from S. antibioticus under a strong promoter [27].