Table 4. Bacterial strains and plasmids used in this study.
| Designation | Characteristics/genotype | Source/Reference |
| A. Bacterial strains | ||
| E. coli ET12567/pUZ8002 | Non-methylating plasmid donor for intergeneric conjugation with Streptomyces | [60] |
| S. antibioticus IMRU 3720 | Wild type | [57] |
| YU5 | ΔmelC mutant | [10] |
| YU5-117 | YU5 containing pSET152-117 inserted at the ΦC31 attB site | This study |
| S. avermitilis | Wild type | Haruo Ikeda |
| B19 | ΔmelC mutant | This study |
| S. coelicolor A3 (2) | ||
| M145 | Prototrophic, SCP1− SCP2− | [59] |
| HY2 | M145 with a his6-tagged melD2 | This study |
| HY69 | ΔmelD mutant of M145 | This study |
| S. lipmanii ATCC 27357 | Wild type | CCRC* |
| S. lividans 1326 | Wild type, SLP2+, SLP3+ | [58] |
| S. maritimus | Wild type | [55] |
| S. tanashiensis ATCC 23967 | Wild type | [55] |
| S. rochei 7434-AN4 | Wild type | [56] |
| B. Plasmids | ||
| pIJ702-117 | Derivative of pIJ702 containing melC of S. antibioticus under a strong promoter | [27] |
| pSET152-117 | Derivative of pSET152 containing melC of pIJ702-117 | This study |
| pLUS702melD | pIJ702-117 with melC substituted by melD and an inserted aac 3(IV) | This study |
| pCR2.1TOPO | E. coli cloning vectors, kanamycin and ampicillin resistance, lacZ α | Invitrogen |
*
Culture Collection and Research Center (CCRC), Food Industry Research and Development Institute, Hsinchu, Taiwan