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. 1998 May 26;95(11):6067–6072. doi: 10.1073/pnas.95.11.6067

Figure 4.

Figure 4

Primer extension experiment showing the abundance of various RNAI-related species in different cultures. Lanes: 1, strain ENS304, pcnB80 derivative; 2 and 3, strain ENS304, no treatment or 20-min kasugamycin treatment, respectively; 4 and 5, strain ENS32 harboring plasmid pBR322, no treatment or 20-min kasugamycin treatment, respectively. The primer used is shown on Fig. 3a. The sequence lane has been generated from pBR322 with the same primer, allowing the immediate assignment of the different RNAI-related species. Spots corresponding to RNAI+31, RNAI, RNAI−5, and RNAI−34 are indicated by arrows. Note that signals from the single-copy RNAI gene in the ENS304 strain (lanes 2 and 3) match in intensity those generated from the plasmid-borne RNAI gene (lanes 3 and 4), reflecting the unique strength of the PT7 promoter (19).