Figure 5.
(a) Effect of addition of chloramphenicol (Left) or kasugamycin (Right) upon accumulation of RNAI+31 and RNAI−5. At the indicated time after drug addition, total RNA was extracted from ENS304 cells and analyzed on Northern blots. (b) Northern blot analysis of the decay of RNA+31 and RNA−5 in control cells (Left) or in cells that have been treated with chloramphenicol for 20 min (Right). At zero time, transcription is switched off by transferring the cells into an identical medium lacking IPTG. All symbols are as in Fig. 3b.