Skip to main content
. 1998 May 26;95(11):6067–6072. doi: 10.1073/pnas.95.11.6067

Figure 6.

Figure 6

(Left) Northern blot showing the accumulation of lacZΔ mRNA in ENS32 cells harboring either the RNase E-overexpressing plasmid (pGM102) or the parent plasmid pET11a (control). Transcription from the plasmid (and from the lacZΔ gene) was induced 30 min before kasugamycin addition. The RNA was analyzed just before (“0”) or 60 min after (“60”) this addition. RNA from the rne-1 derivative of ENS32 (18) harboring pET11a and grown in the absence of kasugamycin at the semi-permissive temperature of 37°C also is shown as a control (fifth lane). Note the stabilization of the lacZΔ mRNA in this strain as compared with the rne+ strain (first lane). (Right) Western blot showing the accumulation of RNase E in the same samples used in Left (0 time). Note the accumulation of RNase E in the rne-1 strain, reflecting loss of autocontrol.